Biomarker Evaluated for Early Detection of Congenital Heart Disease
|
By LabMedica International staff writers Posted on 16 Dec 2020 |
|
Image: The GSP instrument is a high throughput batch analyzer intended for quantitative or qualitative measurement of neonatal screening samples on 96-well microplates (Photo courtesy of PerkinElmer).
Congenital heart disease (CHD) is the most common congenital malformation in humans worldwide. Circulating cardiovascular biomarkers could potentially improve the early detection of CHD, even in asymptomatic newborns.
The amino terminal fragment of the prohormone brain-type natriuretic peptide (NT-proBNP) test has been used in adults with cardiac failure. Evidence has found that the NT-proBNP test is useful in infants who require neonatal intensive care, children with pulmonary hypertension and cardiomyopathies, and children with various types of CHD.
Pediatricians at the University Teaching Hospital Ryhov (Jönköping, Sweden) and their colleagues prospectively enrolled healthy, term neonates born between July 1, 2018, and May 31, 2019 and compared them against retrospectively identified newborns with CHD born between September 1, 2003, and September 30, 2019. The CHD diagnosis was confirmed by echocardiography. Control newborns were followed-up for a minimum of one year using electronic patient records covering routine checkups and additional medical consultations.
The scientists developed a fully automated immunoassay for NT-proBNP measurement from dried blood spot (DBS) samples. Microtitration strips 96-well format were coated with anti-NT-proBNP mouse monoclonal IgG antibody (HyTest Ltd, Turku, Finland) and the monoclonal tracer antibody (HyTest Ltd) was labeled with europium chelate. The hematocrit in this mix was checked with a Coulter Ac · T diff Hematology Analyzer (Beckman Coulter, Brea, CA, USA). A high throughput batch analyzer (GSP Instrument; PerkinElmer, Waltham, MA, USA) was used for the plate assays.
Performed as early as two days after birth, a dried blood spot (DBS) test for NT-proBNP flagged 71% of the 34 tested babies with known CHD and 68% of the critical CHD cases. Combining the DBS test with pulse oximetry screening improved CHD detection to 82% and detection of critical CHD to 89%. Babies with CHD had a median NT-proBNP level of 17,240 ng/L based on the DBS test. Healthy controls had significantly lower NT-proBNP levels, with a median of 1,900 ng/L. A comparison of DBS and standard screening for NT-proBNP (laboratory analysis on 500 μL of blood in a collection tube containing EDTA as an anticoagulant) found the two tests to be highly correlated and in good agreement.
The authors concluded that the DBS NT-proBNP test discriminated well between healthy newborns and newborns with various types of CHD, including critical lesions. Additional reference values were established for days two to four of life, reflecting the timing of common newborn screening programs. The study was published on December 2, 2020 in the journal JAMA Network Open.
Related Links:
University Teaching Hospital Ryhov
HyTest Ltd
Beckman Coulter
PerkinElmer
The amino terminal fragment of the prohormone brain-type natriuretic peptide (NT-proBNP) test has been used in adults with cardiac failure. Evidence has found that the NT-proBNP test is useful in infants who require neonatal intensive care, children with pulmonary hypertension and cardiomyopathies, and children with various types of CHD.
Pediatricians at the University Teaching Hospital Ryhov (Jönköping, Sweden) and their colleagues prospectively enrolled healthy, term neonates born between July 1, 2018, and May 31, 2019 and compared them against retrospectively identified newborns with CHD born between September 1, 2003, and September 30, 2019. The CHD diagnosis was confirmed by echocardiography. Control newborns were followed-up for a minimum of one year using electronic patient records covering routine checkups and additional medical consultations.
The scientists developed a fully automated immunoassay for NT-proBNP measurement from dried blood spot (DBS) samples. Microtitration strips 96-well format were coated with anti-NT-proBNP mouse monoclonal IgG antibody (HyTest Ltd, Turku, Finland) and the monoclonal tracer antibody (HyTest Ltd) was labeled with europium chelate. The hematocrit in this mix was checked with a Coulter Ac · T diff Hematology Analyzer (Beckman Coulter, Brea, CA, USA). A high throughput batch analyzer (GSP Instrument; PerkinElmer, Waltham, MA, USA) was used for the plate assays.
Performed as early as two days after birth, a dried blood spot (DBS) test for NT-proBNP flagged 71% of the 34 tested babies with known CHD and 68% of the critical CHD cases. Combining the DBS test with pulse oximetry screening improved CHD detection to 82% and detection of critical CHD to 89%. Babies with CHD had a median NT-proBNP level of 17,240 ng/L based on the DBS test. Healthy controls had significantly lower NT-proBNP levels, with a median of 1,900 ng/L. A comparison of DBS and standard screening for NT-proBNP (laboratory analysis on 500 μL of blood in a collection tube containing EDTA as an anticoagulant) found the two tests to be highly correlated and in good agreement.
The authors concluded that the DBS NT-proBNP test discriminated well between healthy newborns and newborns with various types of CHD, including critical lesions. Additional reference values were established for days two to four of life, reflecting the timing of common newborn screening programs. The study was published on December 2, 2020 in the journal JAMA Network Open.
Related Links:
University Teaching Hospital Ryhov
HyTest Ltd
Beckman Coulter
PerkinElmer
Platinum Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
Spinal Fluid Cell Count Control
Spinalscopics
Latest Clinical Chem. News
- 3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models
- POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection
- Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases
- New Blood Testing Method Detects Potent Opioids in Under Three Minutes
- Wireless Hepatitis B Test Kit Completes Screening and Data Collection in One Step
- Pain-Free, Low-Cost, Sensitive, Radiation-Free Device Detects Breast Cancer in Urine
- Spit Test Detects Breast Cancer in Five Seconds
- Electrochemical Sensors with Next-Generation Coating Advances Precision Diagnostics at POC
- First-Of-Its-Kind Handheld Device Accurately Detects Fentanyl in Urine within Seconds
- New Fluorescent Sensor Array Lights up Alzheimer’s-Related Proteins for Earlier Detection
- Automated Mass Spectrometry-Based Clinical Analyzer Could Transform Lab Testing
- Highly Sensitive pH Sensor to Aid Detection of Cancers and Vector-Borne Viruses
- Non-Invasive Sensor Monitors Changes in Saliva Compositions to Rapidly Diagnose Diabetes
- Breakthrough Immunoassays to Aid in Risk Assessment of Preeclampsia
- Urine Test for Monitoring Changes in Kidney Health Markers Can Predict New-Onset Heart Failure
- AACC Releases Comprehensive Diabetes Testing Guidelines
Channels
Clinical Chemistry
view channel
3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models
Mass spectrometry is a precise technique for identifying the chemical components of a sample and has significant potential for monitoring chronic illness health states, such as measuring hormone levels... Read more.jpg "Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)")
POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection
Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more
Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases
The conventional methods for measuring free cortisol, the body's stress hormone, from blood or saliva are quite demanding and require sample processing. The most common method, therefore, involves collecting... Read more
Molecular Diagnostics
view channel
Unique Autoantibody Signature to Help Diagnose Multiple Sclerosis Years before Symptom Onset
Autoimmune diseases such as multiple sclerosis (MS) are thought to occur partly due to unusual immune responses to common infections. Early MS symptoms, including dizziness, spasms, and fatigue, often... Read more
Blood Test Could Detect HPV-Associated Cancers 10 Years before Clinical Diagnosis
Human papilloma virus (HPV) is known to cause various cancers, including those of the genitals, anus, mouth, throat, and cervix. HPV-associated oropharyngeal cancer (HPV+OPSCC) is the most common HPV-associated... Read more
.jpg "Image: The new urine-based test detects high-grade prostate cancer (Photo courtesy of U-M Rogel Cancer Center)"){kind=small}
Hematology
view channel
Next Generation Instrument Screens for Hemoglobin Disorders in Newborns
Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more
First 4-in-1 Nucleic Acid Test for Arbovirus Screening to Reduce Risk of Transfusion-Transmitted Infections
Arboviruses represent an emerging global health threat, exacerbated by climate change and increased international travel that is facilitating their spread across new regions. Chikungunya, dengue, West... Read more
POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy
Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more
First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy
Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read moreMicrobiology
view channel
Mouth Bacteria Test Could Predict Colon Cancer Progression
Colon cancer, a relatively common but challenging disease to diagnose, requires confirmation through a colonoscopy or surgery. Recently, there has been a worrying increase in colon cancer rates among younger... Read more.jpg "Image: Dr. Sam Lodge found that metabolic signatures can shift the speed of sepsis diagnosis (Photo courtesy of Murdoch University)")
Unique Metabolic Signature Could Enable Sepsis Diagnosis within One Hour of Blood Collection
Sepsis is a life-threatening condition triggered by an extreme response of the body to an infection. It requires immediate medical intervention to prevent potential death or lasting damage.... Read more
Pathology
view channel
Spatial Tissue Analysis Identifies Patterns Associated With Ovarian Cancer Relapse
High-grade serous ovarian carcinoma is the most lethal type of ovarian cancer, and it poses significant detection challenges. Typically, patients initially respond to surgery and chemotherapy, but the... Read more.jpg "Image: Fingertip blood sample collection on the Babson Handwarmer (Photo courtesy of Babson Diagnostics)")
Unique Hand-Warming Technology Supports High-Quality Fingertip Blood Sample Collection
Warming the hand is an effective way to facilitate blood collection from a fingertip, yet off-the-shelf solutions often do not fulfill laboratory requirements. Now, a unique hand-warming technology has... Read more
Technology
view channel
New Diagnostic System Achieves PCR Testing Accuracy
While PCR tests are the gold standard of accuracy for virology testing, they come with limitations such as complexity, the need for skilled lab operators, and longer result times. They also require complex... Read more
DNA Biosensor Enables Early Diagnosis of Cervical Cancer
Molybdenum disulfide (MoS2), recognized for its potential to form two-dimensional nanosheets like graphene, is a material that's increasingly catching the eye of the scientific community.... Read more
Self-Heating Microfluidic Devices Can Detect Diseases in Tiny Blood or Fluid Samples
Microfluidics, which are miniature devices that control the flow of liquids and facilitate chemical reactions, play a key role in disease detection from small samples of blood or other fluids.... Read more
Breakthrough in Diagnostic Technology Could Make On-The-Spot Testing Widely Accessible
Home testing gained significant importance during the COVID-19 pandemic, yet the availability of rapid tests is limited, and most of them can only drive one liquid across the strip, leading to continued... Read moreIndustry
view channel
ECCMID Congress Name Changes to ESCMID Global
Over the last few years, the European Society of Clinical Microbiology and Infectious Diseases (ESCMID, Basel, Switzerland) has evolved remarkably. The society is now stronger and broader than ever before... Read more
Bosch and Randox Partner to Make Strategic Investment in Vivalytic Analysis Platform
Given the presence of so many diseases, determining whether a patient is presenting the symptoms of a simple cold, the flu, or something as severe as life-threatening meningitis is usually only possible... Read more
Siemens to Close Fast Track Diagnostics Business
Siemens Healthineers (Erlangen, Germany) has announced its intention to close its Fast Track Diagnostics unit, a small collection of polymerase chain reaction (PCR) testing products that is part of the... Read more